Membrane filtration of Sudan orange G on a cellulose acetate membrane filter for separation-preconcentration and spectrophotometric determination in water, chili powder, chili sauce and tomato sauce samples

Food Chem Toxicol. 2012 Aug;50(8):2709-13. doi: 10.1016/j.fct.2012.05.021. Epub 2012 May 19.

Abstract

A simple membrane filtration procedure for separation-enrichment of Sudan orange G is presented. The method is based on the adsorption of Sudan orange G on a cellulose acetate filter and its elution from the membrane with 10 mL of ethanol. Sudan orange G in the eluent was determined by UV-visible spectrophotometry at 388 nm. The effect of analytical conditions, including pH, flow rates and eluent, sample volume, type of membrane for quantitative preconcentration and separation of Sudan orange G were examined. The influences of matrix components on Sudan orange G recoveries were studied. The preconcentration factor was 125. The detection limit was 4.9 μg L(-1). The relative standard deviation was 4.3%. The presented procedure was applied to chili powder, chili sauce, tomato sauce, powdered beverage and water samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellulose / analogs & derivatives*
  • Cellulose / chemistry
  • Coloring Agents / isolation & purification*
  • Filtration / methods*
  • Food Analysis*
  • Hydrogen-Ion Concentration
  • Membranes, Artificial*
  • Spectrophotometry, Ultraviolet / methods*

Substances

  • Coloring Agents
  • Membranes, Artificial
  • acetylcellulose
  • Cellulose