Microsatellite markers for Bistorta vivipara (Polygonaceae)

Abstract

Premise of the study: Using genomic shotgun 454 sequencing, 50 candidate microsatellite markers were targeted for the arctic-alpine polyploid perennial herb Bistorta vivipara to distinguish between individual genets and ramets within a population.

Methods and results: Out of the 50 markers, 31 were polymorphic for seven test samples. We have developed a multiplex protocol for 16 of these microsatellite markers.

Conclusions: Our results show that the microsatellite markers provide a powerful tool for the research on genetic variation of B. vivipara.