Complete nucleotide sequence of a new strain of grapevine leafroll-associated virus 3 in South Africa

R Bester; H J Maree; J T Burger

doi:10.1007/s00705-012-1333-8

Complete nucleotide sequence of a new strain of grapevine leafroll-associated virus 3 in South Africa

Arch Virol. 2012 Sep;157(9):1815-9. doi: 10.1007/s00705-012-1333-8. Epub 2012 May 22.

Authors

R Bester¹, H J Maree, J T Burger

Affiliation

¹ Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa.

PMID: 22614811
DOI: 10.1007/s00705-012-1333-8

Abstract

The complete genome sequences of two newly identified genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) are described. Two isolates, GH11 (18671 nt) and GH30 (18576 nt) were sequenced and found to be less than 70 % similar to other South African GLRaV-3 variants. The genome organization of GH11 and GH30 is similar to that of previously described GLRaV-3 isolates. However, no corresponding open reading frame 2 (ORF2) could be identified. Phylogenetic analysis indicates that GH11 and GH30 cluster in a subgroup (group VI) that is separate from the previously described GLRaV-3 isolates and should be regarded as a different strain of GLRaV-3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Closteroviridae / classification
Closteroviridae / genetics*
Closteroviridae / isolation & purification*
Cluster Analysis
Gene Order
Genome, Viral*
Molecular Sequence Data
Open Reading Frames
Phylogeny
Plant Diseases / virology*
RNA, Viral / genetics*
Sequence Analysis, DNA
South Africa
Synteny
Vitis / virology*

Substances

RNA, Viral

Associated data

GENBANK/JQ655295
GENBANK/JQ655296