De novo sequencing and analysis of the termite mushroom (Termitomyces albuminosus) transcriptome to discover putative genes involved in bioactive component biosynthesis

Fang Yang; Bo Xu; Sanjun Zhao; Junjun Li; Yunjuan Yang; Xianghua Tang; Feng Wang; Mozhen Peng; Zunxi Huang

doi:10.1016/j.jbiosc.2012.03.009

De novo sequencing and analysis of the termite mushroom (Termitomyces albuminosus) transcriptome to discover putative genes involved in bioactive component biosynthesis

J Biosci Bioeng. 2012 Aug;114(2):228-31. doi: 10.1016/j.jbiosc.2012.03.009. Epub 2012 May 17.

Authors

Fang Yang¹, Bo Xu, Sanjun Zhao, Junjun Li, Yunjuan Yang, Xianghua Tang, Feng Wang, Mozhen Peng, Zunxi Huang

Affiliation

¹ School of Life Science, Yunnan Normal University, Kunming 650500, China.

PMID: 22608552
DOI: 10.1016/j.jbiosc.2012.03.009

Abstract

A one-eighth 454 sequencing run produced 82,386 high-quality reads. De novo assembly generated 6494 unique sequences. Based on the bioinformatic analysis, we found many the known enzymes involved in the biosynthesis of triterpene saponin in Termitomyces albuminosus, including 6 cytochrome P450 and 22 glycosyltransferase unique genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cytochrome P-450 Enzyme System / genetics
Expressed Sequence Tags
Ginsenosides / biosynthesis
Ginsenosides / chemistry
Glycosyltransferases / genetics
Saponins / biosynthesis*
Sequence Analysis, DNA
Termitomyces / enzymology
Termitomyces / genetics*
Termitomyces / metabolism*
Transcriptome / genetics*
Triterpenes / chemistry
Triterpenes / metabolism*

Substances

Ginsenosides
Saponins
Triterpenes
Cytochrome P-450 Enzyme System
Glycosyltransferases