Gel drying methods

Methods Mol Biol. 2012:869:433-6. doi: 10.1007/978-1-61779-821-4_36.

Abstract

For some instances, protein gels need to be dried after SDS-PAGE, for example, if autoradiography should be performed from radioactive-labeled proteins after their separation on SDS-polyacrylamide gels. Another reason may be to simply store the gel in the laboratory book. Aside from expensive commercial solutions, especially for storage of the dried gel in the lab book, the simple and cheap drying protocol here presented may be sufficient.

MeSH terms

  • Buffers
  • Cellophane / chemistry
  • Desiccation / instrumentation
  • Desiccation / methods*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Proteins / chemistry
  • Proteins / isolation & purification

Substances

  • Buffers
  • Proteins
  • Cellophane