Abstract
Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Outer Membrane Proteins / chemistry*
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Bacterial Outer Membrane Proteins / metabolism*
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Bacteriophages / chemistry*
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Bacteriophages / metabolism*
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Binding Sites
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Escherichia coli / chemistry*
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Escherichia coli / metabolism*
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Escherichia coli Proteins / chemistry*
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Escherichia coli Proteins / metabolism*
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T-Phages / chemistry*
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T-Phages / metabolism
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Viral Proteins / chemistry*
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Viral Proteins / metabolism*
Substances
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Bacterial Outer Membrane Proteins
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Escherichia coli Proteins
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FhuA protein, E coli
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Viral Proteins