Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Analyst. 2012 Jul 21;137(14):3227-9. doi: 10.1039/c2an35138b. Epub 2012 May 11.

Abstract

Here we report a novel method for the manipulation and concentration of Aβ amyloid fibrils, implicated in Alzheimer's disease, using DC insulating gradient dielectrophoresis (DC-iGDEP). Fibril enrichment was found to be ∼400%. Simulations suggest that capture of the full range of amyloid protein aggregates is possible with optimized device design.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amyloid beta-Peptides / chemistry*
  • Amyloid beta-Peptides / isolation & purification*
  • Electric Conductivity*
  • Electric Impedance
  • Electrophoresis / methods*
  • Protein Multimerization*
  • Protein Structure, Secondary

Substances

  • Amyloid beta-Peptides