Chloroplastic NADPH-dependent alkenal/one oxidoreductase contributes to the detoxification of reactive carbonyls produced under oxidative stress

FEBS Lett. 2012 Apr 24;586(8):1208-13. doi: 10.1016/j.febslet.2012.03.013. Epub 2012 Mar 17.

Abstract

Lipid peroxide-derived reactive carbonyls (RCs) can cause serious damage to plant functions. A chloroplastic NADPH-dependent alkenal/one oxidoreductase (AOR) detoxifies RCs, but its physiological significance remains unknown. In this study, we investigated the biological impacts of AOR using an AOR-knock out Arabidopsis line (aor). Methyl viologen treatment, mainly to enhance photosystem (PS) I-originated reactive oxygen species (ROS) production, caused more severe damage to aor than wild type (Col-0). In contrast, the high light treatment used to enhance PSII-originated ROS production resulted in no difference in PSII damage between Col-0 and aor. In conclusion, AOR can contribute to detoxify stromal RCs produced under oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / genetics
  • Alcohol Oxidoreductases / metabolism*
  • Arabidopsis / enzymology
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Chloroplasts / enzymology
  • Chloroplasts / metabolism*
  • Gene Knockout Techniques
  • Oxidative Stress*
  • Oxidoreductases Acting on Aldehyde or Oxo Group Donors / genetics
  • Oxidoreductases Acting on Aldehyde or Oxo Group Donors / metabolism*
  • Photosystem I Protein Complex / metabolism
  • Reactive Oxygen Species / metabolism

Substances

  • Arabidopsis Proteins
  • Photosystem I Protein Complex
  • Reactive Oxygen Species
  • Alcohol Oxidoreductases
  • leukotriene B4 12-hydroxydehydrogenase
  • AOR protein, Arabidopsis
  • Oxidoreductases Acting on Aldehyde or Oxo Group Donors