Ovine scrapie and cervid chronic wasting disease can be transmitted in the absence of animal-to-animal contact, and environmental reservoirs of infectivity have been implicated in their spread and persistence. Investigating environmental factors that influence the interaction of disease-associated PrP with soils is imperative to understanding what is likely to be the complex role of soil in disease transmission. Here, we describe the effects of soil temperature on the binding/desorption and persistence of both ovine scrapie- and bovine BSE-PrP (TSE) . Binding of PrP (TSE) to a sandy loam soil at temperatures of 4°C, 8-12°C and 25-30°C demonstrated that an increase in temperature resulted in (1) a decrease in the amount of PrP (TSE) recovered after 24 h of interaction with soil, (2) an increase in the amount of N-terminal cleavage of the prion protein over 11 d and (3) a decrease in the persistence of PrP (TSE) on soil over an 18 mo period.