Note: multiplexed multiple-tau auto- and cross-correlators on a single field programmable gate array

G Mocsár; B Kreith; J Buchholz; J W Krieger; J Langowski; G Vámosi

doi:10.1063/1.3700810

Note: multiplexed multiple-tau auto- and cross-correlators on a single field programmable gate array

Rev Sci Instrum. 2012 Apr;83(4):046101. doi: 10.1063/1.3700810.

Authors

G Mocsár¹, B Kreith, J Buchholz, J W Krieger, J Langowski, G Vámosi

Affiliation

¹ Department of Biophysics and Cell Biology, Medical and Health Science Center, University of Debrecen, Debrecen 4032, Hungary.

PMID: 22559587
DOI: 10.1063/1.3700810

Abstract

We introduce a new multiple-tau hardware correlator design for computing fluorescence correlation functions (CFs) in real time. Use of hardware resources is minimized by scheduling the computation of different segments of the CFs on a single correlator block. Simultaneous calculation of two multiple-tau autocorrelation (ACFs) and two cross-correlation functions (CCFs) is implemented in LabVIEW on a National Instruments field programmable gate array (FPGA) card with a minimal sampling time of 400 ns. Raw data are stored with a time resolution of 50 ns. The design can be easily adapted to other FPGA cards and extended to more inputs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Models, Theoretical
Reproducibility of Results
Spectrometry, Fluorescence / instrumentation*
Time Factors