Visible light-driven NADH regeneration sensitized by proflavine for biocatalysis

Dong Heon Nam; Chan Beum Park

doi:10.1002/cbic.201200115

Visible light-driven NADH regeneration sensitized by proflavine for biocatalysis

Chembiochem. 2012 Jun 18;13(9):1278-82. doi: 10.1002/cbic.201200115. Epub 2012 May 3.

Authors

Dong Heon Nam¹, Chan Beum Park

Affiliation

¹ KAIST Institute for the BioCentury, Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), 335 Science Road, Daejeon 305-701, South Korea.

PMID: 22555876
DOI: 10.1002/cbic.201200115

Abstract

Harvest time: Proflavine drives the reduction of NAD(+) in the presence of a Rh-based electron mediator. Photoregenerated NADH was enzymatically active for oxidation by NADH-dependent L-glutamate dehydrogenase for the synthesis of L-glutamate. This work suggests that proflavine has the potential to become an efficient light-harvesting component in biocatalytic photosynthesis driven by solar energy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocatalysis / drug effects*
Biocatalysis / radiation effects*
Glutamic Acid / biosynthesis
Light*
NAD / metabolism*
Photosensitizing Agents / pharmacology*
Photosynthesis / drug effects
Photosynthesis / radiation effects
Proflavine / pharmacology*

Substances

Photosensitizing Agents
NAD
Glutamic Acid
Proflavine