Confocal laser scanning microscopy for the study of the morphological changes of the postextraction sites

Microsc Res Tech. 2012 Apr;75(4):513-9. doi: 10.1002/jemt.21085.

Abstract

A better understanding of the remodeling process of postextraction sockets is essential in dental treatment planning. The aim of this study was to evaluate whether confocal laser scanning microscopy (CLSM) can be applied to imaging contour changes of postextraction sites, as well as to its quantification with image analysis of obtained three-dimensional images. This work describes a new application of the CLSM technique. The system used was the OLS3100-USS, LEXT model (Olympus®). CLSM was used for the surface analysis of the extraction site. The measurements taken with CLSM were: (1) mesio-distal distance, (2) alveolar ridge thickness, and (3) vestibular and lingual alveolar ridge height. Results of study cast scanning at baseline, 1 and 3 months after tooth extraction, with CLSM are well-detailed images of postextraction areas. The CLSM technique used in study casts is a valid method to measure the dimensional changes that happen in the edentulous area after tooth extraction. This technique allows the evaluation of changes in mesio-distal distance, thickness of the alveolar ridge and alveolar ridge height based on the measurements on the alveolar contours.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alveolar Process / anatomy & histology*
  • Humans
  • Imaging, Three-Dimensional / methods*
  • Microscopy, Confocal / methods*
  • Statistics, Nonparametric
  • Tooth Extraction*
  • Tooth Socket / anatomy & histology