Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus

Piyathida Pongsiri; Kesmanee Praianantathavorn; Apiradee Theamboonlers; Sunchai Payungporn; Yong Poovorawan

doi:10.1016/S1995-7645(12)60055-8

Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus

Asian Pac J Trop Med. 2012 May;5(5):342-6. doi: 10.1016/S1995-7645(12)60055-8.

Authors

Piyathida Pongsiri¹, Kesmanee Praianantathavorn, Apiradee Theamboonlers, Sunchai Payungporn, Yong Poovorawan

Affiliation

¹ Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Abstract

Objective: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.

Methods: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.

Results: The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.

Conclusions: This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Alphavirus Infections / diagnosis*
Chikungunya Fever
Chikungunya virus / isolation & purification*
DNA Probes
Dengue / diagnosis*
Dengue Virus / isolation & purification*
Female
Humans
Male
Nucleic Acid Amplification Techniques
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity

Substances

DNA Probes