Abstract
Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde-Lyases / genetics
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Aldehyde-Lyases / metabolism*
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Arabidopsis / enzymology
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Arabidopsis / genetics*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Bioreactors
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Biotechnology / methods*
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Nitriles / chemistry
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Nitriles / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism*
Substances
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Arabidopsis Proteins
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Nitriles
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Recombinant Fusion Proteins
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cyanohydrin
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Aldehyde-Lyases
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mandelonitrile lyase