Abstract
A novel rutin-α-L-rhamnosidase hydrolyzing α-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the α-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Aspergillus niger / enzymology*
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Aspergillus niger / genetics
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Aspergillus niger / metabolism
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Cloning, Molecular
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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Flavanones / metabolism*
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Gene Expression
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Glycoside Hydrolases / genetics*
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Glycoside Hydrolases / isolation & purification
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Glycoside Hydrolases / metabolism*
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Hesperidin / metabolism*
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Molecular Sequence Data
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Pichia / genetics
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Rutin / metabolism*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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DNA, Fungal
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Flavanones
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Rutin
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Hesperidin
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Glycoside Hydrolases
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naringin