An improved medium for growing Staphylococcus aureus biofilm

Ping Chen; Johnathan J Abercrombie; Nicole R Jeffrey; Kai P Leung

doi:10.1016/j.mimet.2012.04.009

An improved medium for growing Staphylococcus aureus biofilm

J Microbiol Methods. 2012 Aug;90(2):115-8. doi: 10.1016/j.mimet.2012.04.009. Epub 2012 Apr 19.

Authors

Ping Chen¹, Johnathan J Abercrombie, Nicole R Jeffrey, Kai P Leung

Affiliation

¹ Microbiology Branch, US Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, TX 78234, USA. ping.chen@us.army.mil

PMID: 22542521
DOI: 10.1016/j.mimet.2012.04.009

Abstract

A medium (Brain Heart Infusion plus 10% human plasma) was developed, tested, and validated for growing Staphylococcus aureus biofilm in vitro. With this medium, S. aureus forms reproducible and robust biofilms in flow chambers under controlled shear flow and with increased viability recovery in static well plates.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Validation Study

MeSH terms

Bacteriological Techniques / methods*
Biofilms / growth & development*
Culture Media / chemistry*
Humans
Microbial Viability
Plasma / metabolism
Staphylococcus aureus / growth & development
Staphylococcus aureus / physiology*

Substances

Culture Media