Calf thymus DNA binding studies of the new neodymium-naproxen complex

Ruina Huo; Guiqing Xu; Xiaoying Jiang; Yao Ge; Zaikun Xue; Fengling Cui

doi:10.1002/jbt.21401

Calf thymus DNA binding studies of the new neodymium-naproxen complex

J Biochem Mol Toxicol. 2012 May;26(5):193-8. doi: 10.1002/jbt.21401. Epub 2012 Apr 26.

Authors

Ruina Huo¹, Guiqing Xu, Xiaoying Jiang, Yao Ge, Zaikun Xue, Fengling Cui

Affiliation

¹ School of Chemistry and Environmental Sciences, Henan Normal University, Xinxiang 453007, People's Republic of China.

PMID: 22539453
DOI: 10.1002/jbt.21401

Abstract

Fluorescence spectroscopy in combination with UV absorption spectroscopy was carried out to investigate the interaction between the neodymium-naproxen complex (Nd-NAP) and calf thymus DNA (ctDNA). The experimental results showed that Nd-NAP intercalated with the ctDNA base pairs. Analysis of fluorescence quenching data of Nd-NAP by ctDNA at different temperatures using a Stern-Volmer equation revealed that dynamic and static quenching occurred simultaneously. The binding constants and the number of binding sites at 293 and 310 K were obtained as 2.904 × 10(4) L mol(-1), 1.172 and 2.432 × 10(4) L mol(-1), 1.143, respectively. The thermodynamic parameters ΔG, ΔH, and ΔS calculated at different temperatures indicated that hydrogen bonding and van der Waals force were the main binding forces.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
DNA / chemistry*
Naproxen* / chemistry
Neodymium* / chemistry
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Thermodynamics

Substances

Neodymium
Naproxen
DNA
calf thymus DNA