Impact of H-aggregation on activatable MMP-2-specific probes for optical imaging

Contrast Media Mol Imaging. 2012 May-Jun;7(3):328-37. doi: 10.1002/cmmi.500.

Abstract

In order to target and image MMP-2 activity using optical imaging, we developed a panel of new MMP-2 probes based on Cy5 and QSY21 as fluorophore/quencher FRET partners, separated by various MMP-2 specific peptide substrates. We compared these probes for their specificity against other MMPs, their rate of activation by MMP-2 and their initial quenching.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbocyanines*
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes*
  • Humans
  • Mass Spectrometry
  • Matrix Metalloproteinase 2 / metabolism*
  • Matrix Metalloproteinases / analysis
  • Matrix Metalloproteinases / chemistry
  • Matrix Metalloproteinases / metabolism*
  • Molecular Imaging / methods*
  • Molecular Probes*
  • Peptide Fragments / metabolism*

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • Molecular Probes
  • Peptide Fragments
  • cyanine dye 5
  • Matrix Metalloproteinases
  • Matrix Metalloproteinase 2