Abstract
In order to target and image MMP-2 activity using optical imaging, we developed a panel of new MMP-2 probes based on Cy5 and QSY21 as fluorophore/quencher FRET partners, separated by various MMP-2 specific peptide substrates. We compared these probes for their specificity against other MMPs, their rate of activation by MMP-2 and their initial quenching.
Copyright © 2012 John Wiley & Sons, Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbocyanines*
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes*
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Humans
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Mass Spectrometry
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Matrix Metalloproteinase 2 / metabolism*
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Matrix Metalloproteinases / analysis
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Matrix Metalloproteinases / chemistry
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Matrix Metalloproteinases / metabolism*
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Molecular Imaging / methods*
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Molecular Probes*
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Peptide Fragments / metabolism*
Substances
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Carbocyanines
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Fluorescent Dyes
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Molecular Probes
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Peptide Fragments
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cyanine dye 5
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Matrix Metalloproteinases
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Matrix Metalloproteinase 2