Abstract
Clostridium botulinum type B is estimated to cause more than 85% of cases of equine botulism in the United States, as well as many outbreaks in cattle. In this study, a quantitative real-time polymerase chain reaction for detection of the neurotoxin gene of C. botulinum type B was compared to the mouse bioassay using 45 positive and 43 negative samples of equine, bovine or associated environmental origin. The sensitivity of the qPCR assay was 96%, whereas the sensitivity of the mouse bioassay was 84%. The specificity of the qPCR assay was 95% and the specificity of the mouse bioassay was 100%.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biological Assay / methods
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Botulinum Toxins / genetics
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Botulinum Toxins / metabolism*
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Botulinum Toxins, Type A
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Botulism / diagnosis*
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Botulism / microbiology
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Cattle
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Cattle Diseases / diagnosis*
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Cattle Diseases / microbiology
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Clostridium botulinum / classification*
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Horse Diseases / diagnosis*
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Horse Diseases / microbiology
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Horses
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Mice
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Real-Time Polymerase Chain Reaction / methods
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Real-Time Polymerase Chain Reaction / veterinary*
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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rimabotulinumtoxinB
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Botulinum Toxins
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Botulinum Toxins, Type A