Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species

J Clin Microbiol. 2012 Jul;50(7):2479-83. doi: 10.1128/JCM.00224-12. Epub 2012 Apr 25.

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology*
  • Aspergillus / drug effects*
  • Candida / drug effects*
  • Caspofungin
  • Echinocandins / pharmacology*
  • Humans
  • Lipopeptides
  • Microbial Sensitivity Tests / methods
  • Mutation
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Antifungal Agents
  • Echinocandins
  • Lipopeptides
  • Caspofungin