In this study, we found that almost all institutions conducting animal experiments, such as universities, corporations, and research laboratories, also conducted memorial services for the animals sacrificed during animal experimentation. A questionnaire survey was conducted among 120 institutions. A total of 83 (69.1%) valid responses were obtained from the participating institutions. Memorial services were held at 79 institutions (95.1%). Memorial services for laboratory animals have been mainly conducted to show appreciation, comfort the spirit, and console the souls.