Abstract
Neurotoxicity in Alzheimer's disease (AD) is associated to dishomeostasis of intracellular Ca(2+) induced by amyloid β peptide (Aβ) species. Understanding of the effects of Aβ on intracellular Ca(2+) homeostasis requires preparation of the different Aβ assemblies including oligomers and fibrils and the testing of their effects on cytosolic and mitochondrial Ca(2+) in neurons. Procedures for cerebellar granule cell culture, preparation of Aβ species as well as fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca(2+) in neurons are described.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid beta-Peptides / chemistry
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Amyloid beta-Peptides / toxicity*
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Animals
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Calcium / metabolism
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Calcium Signaling / drug effects*
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Cells, Cultured
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Cytosol / drug effects
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Cytosol / metabolism
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Fura-2 / metabolism
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Intracellular Space / drug effects*
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Intracellular Space / metabolism*
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Luminescent Measurements
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Mitochondria / drug effects
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Mitochondria / metabolism
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Molecular Imaging
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Neurons / cytology*
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Neurons / drug effects*
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Neurons / metabolism
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Peptide Fragments / chemistry
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Peptide Fragments / toxicity*
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Protein Multimerization
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Protein Structure, Secondary
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Rats
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Rats, Wistar
Substances
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Amyloid beta-Peptides
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Peptide Fragments
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amyloid beta-protein (1-42)
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Calcium
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Fura-2