High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Siu F Lee; Vanessa L White; Andrew R Weeks; Ary A Hoffmann; Nancy M Endersby

doi:10.1128/AEM.00069-12

High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Appl Environ Microbiol. 2012 Jul;78(13):4740-3. doi: 10.1128/AEM.00069-12. Epub 2012 Apr 20.

Authors

Siu F Lee¹, Vanessa L White, Andrew R Weeks, Ary A Hoffmann, Nancy M Endersby

Affiliation

¹ Department of Genetics, Bio21 Institute, The University of Melbourne, Victoria, Australia. ronaldl@unimelb.edu.au

Abstract

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Aedes / microbiology*
Animals
Drosophila / microbiology*
Fluorescence
High-Throughput Screening Assays / economics
High-Throughput Screening Assays / methods*
Polymerase Chain Reaction / economics
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Wolbachia / genetics*
Wolbachia / isolation & purification*