Abstract
We examined the receptor-mediated effects of extracellular ATP on neuronal differentiation of PC12 cells, Neuro2a cells and MEB5 cells by using a series of receptor antagonists. The P2Y13 receptor antagonist MRS2211 significantly accelerated neurite outgrowth in all cases. Treatment with nerve growth factor (NGF) alone activated ERK1/2 in PC12 cells, and the activation was further increased by MRS2211. These results suggest involvement of P2Y13 receptor in suppression of neuronal differentiation. Thus, P2Y13 receptor antagonists might be candidates for treatment of neurodegenerative diseases.
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
MeSH terms
-
Animals
-
Azo Compounds / pharmacology
-
Cell Differentiation* / drug effects
-
Cell Line
-
Mice
-
Mitogen-Activated Protein Kinase 3 / metabolism
-
Mitogen-Activated Protein Kinases / metabolism
-
Nerve Growth Factor / pharmacology
-
Neurons / cytology*
-
Neurons / drug effects
-
Neurons / metabolism
-
Pyridoxal Phosphate / analogs & derivatives
-
Pyridoxal Phosphate / pharmacology
-
Rats
-
Receptors, Purinergic P2 / metabolism*
-
Signal Transduction / drug effects
Substances
-
Azo Compounds
-
MRS 2211
-
P2ry13 protein, mouse
-
P2ry13 protein, rat
-
Receptors, Purinergic P2
-
Pyridoxal Phosphate
-
Nerve Growth Factor
-
Mitogen-Activated Protein Kinase 3
-
Mitogen-Activated Protein Kinases