Validation of increased blood storage times with the T-SPOT.TB assay with T-Cell Xtend reagent in individuals with different tuberculosis risk factors

J Clin Microbiol. 2012 Jul;50(7):2469-71. doi: 10.1128/JCM.00674-12. Epub 2012 Apr 18.

Abstract

We compared the performance of the T-SPOT.TB assay with blood used within 0 to 3.5 h after collection (control) to its performance with blood stored for 0 to 3.5, 5 to 8, 18 to 21, or 31 to 33 h with the addition of T-Cell Xtend (experimental), using samples from 154 participants. The 95.4% concordance between paired specimens indicated that blood can be stored for up to 33 h prior to T-SPOT.TB testing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blood / immunology*
  • Female
  • Humans
  • Indicators and Reagents / chemistry
  • Interferon-gamma Release Tests / methods*
  • Male
  • Middle Aged
  • Specimen Handling / methods*
  • Time Factors
  • Tuberculosis / diagnosis*
  • Tuberculosis / microbiology

Substances

  • Indicators and Reagents