Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

Chem Commun (Camb). 2012 May 25;48(42):5127-9. doi: 10.1039/c2cc30373f. Epub 2012 Apr 19.

Abstract

A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Hydrogen-Ion Concentration
  • Luminescent Proteins / chemistry
  • Luminescent Proteins / metabolism*
  • Mice
  • Microscopy, Confocal

Substances

  • Luminescent Proteins