The yields of transcripts for a RNA polymerase regulated by hairpin structures in nascent RNAs

Chem Commun (Camb). 2012 May 25;48(42):5121-3. doi: 10.1039/c2cc31657a. Epub 2012 Apr 19.

Abstract

The yields of transcripts capable of forming RNA hairpins with different stem lengths at elongation phase were determined in a T7 RNA polymerase. The amount of RNA transcripts decreased with stem length, demonstrating that transcriptional efficiency is regulated by secondary structure formation in the nascent RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA-Directed RNA Polymerases / metabolism*
  • Inverted Repeat Sequences
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • RNA / chemistry*
  • RNA / metabolism
  • Transcription, Genetic
  • Viral Proteins / metabolism*

Substances

  • Viral Proteins
  • RNA
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases