Abstract
The yields of transcripts capable of forming RNA hairpins with different stem lengths at elongation phase were determined in a T7 RNA polymerase. The amount of RNA transcripts decreased with stem length, demonstrating that transcriptional efficiency is regulated by secondary structure formation in the nascent RNA.
This journal is © The Royal Society of Chemistry 2012
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA-Directed RNA Polymerases / metabolism*
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Inverted Repeat Sequences
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Molecular Sequence Data
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Nucleic Acid Conformation
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RNA / chemistry*
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RNA / metabolism
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Transcription, Genetic
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Viral Proteins / metabolism*
Substances
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Viral Proteins
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RNA
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bacteriophage T7 RNA polymerase
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DNA-Directed RNA Polymerases