Vaccination of mice with recombinant bacille Calmette-Guérin harboring Rv1357c protects similarly to native BCG

Int J Tuberc Lung Dis. 2012 Jun;16(6):774-6. doi: 10.5588/ijtld.11.0735. Epub 2012 Apr 9.

Abstract

Despite the availability of a Mycobacterium bovis bacille Calmette Guérin (BCG) vaccine, tuberculosis (TB) remains a global public health problem. In this study, we introduced the c-di-GMP phosphodiesterase gene Rv1357c, implicated in regulating mycobacterial replication within macrophages, into BCG Pasteur, and tested the resulting strain for its capacity to serve as a vaccine against TB in a murine model. Modified BCG was more phagocytosed than its parental strain, but halted bacterial replication, and protected against M. tuberculosis challenge similarly to unmodified BCG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases / genetics
  • Acyltransferases / immunology*
  • Animals
  • BCG Vaccine / immunology*
  • Cell Line
  • Disease Models, Animal
  • Macrophages / immunology
  • Macrophages / microbiology
  • Mice
  • Mice, Inbred BALB C
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / immunology*
  • Phagocytosis
  • Time Factors
  • Tuberculosis, Pulmonary / immunology
  • Tuberculosis, Pulmonary / microbiology
  • Tuberculosis, Pulmonary / prevention & control*
  • Vaccination*
  • Vaccines, Synthetic / immunology

Substances

  • BCG Vaccine
  • Vaccines, Synthetic
  • Acyltransferases
  • Rv1347c, Mycobacterium tuberculosis