Synthesis of a 3-deoxy-D-manno-octulosonic acid (KDO) building block from D-glucose via fermentation

Gulden Camci-Unal; Rahman M Mizanur; Yonghai Chai; Nicola L B Pohl

doi:10.1039/c2ob25168j

Synthesis of a 3-deoxy-D-manno-octulosonic acid (KDO) building block from D-glucose via fermentation

Org Biomol Chem. 2012 Aug 14;10(30):5856-60. doi: 10.1039/c2ob25168j. Epub 2012 Apr 16.

Authors

Gulden Camci-Unal¹, Rahman M Mizanur, Yonghai Chai, Nicola L B Pohl

Affiliation

¹ Department of Chemistry, Iowa State University, Ames, IA 50011-3111, USA.

PMID: 22505005
DOI: 10.1039/c2ob25168j

Abstract

Herein we report the first synthesis of a 3-deoxy-D-manno-octulosonic acid (KDO) building block starting from glucose through pathway engineering of Escherichia coli and subsequent chemical modifications to provide an alternative method to produce KDO, found in plant and bacterial oligosaccharides.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Escherichia coli / cytology
Escherichia coli / genetics
Escherichia coli / metabolism
Fermentation*
Genetic Engineering
Glucose / metabolism*
Sugar Acids / metabolism*

Substances

Sugar Acids
2-keto-3-deoxyoctonate
Glucose