Abstract
Nonglycosylated erythropoietin bearing acetamidomethyl protecting groups at the cysteine residues has been synthesized via chemical methods. Alanine ligation was used to assemble four peptide fragments, themselves prepared by solid phase peptide synthesis. This work outlines a route for the synthesis of homogeneous glycosylated erythropoietin.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Alanine / chemistry*
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Amino Acid Sequence
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Carbohydrate Sequence
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Cysteine / chemistry*
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Erythropoietin / chemical synthesis*
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Erythropoietin / chemistry
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Erythropoietin / metabolism
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Glycosylation
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Models, Chemical
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Molecular Sequence Data
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Molecular Structure
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Oligosaccharides / chemistry
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Oligosaccharides / metabolism
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Peptide Fragments / chemistry*
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Peptide Fragments / metabolism
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Protein Stability
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Protein Structure, Secondary
Substances
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Oligosaccharides
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Peptide Fragments
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Erythropoietin
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Cysteine
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Alanine