Structural analysis of the rabbit TNF locus, containing the genes encoding TNF-beta (lymphotoxin) and TNF-alpha (tumor necrosis factor)

Gene. 1990 Nov 15;95(2):215-21. doi: 10.1016/0378-1119(90)90364-w.

Abstract

The genes coding for 20-kDa lymphotoxin (TNF-beta) and tumor necrosis factor (TNF-alpha) have been cloned from a rabbit genomic library. The two genes are tandemly arranged and separated by only 1 kb of DNA, as previously observed in human and mouse genomes. We have sequenced the entire rabbit lymphotoxin-encoding gene and inferred the primary structure of rabbit TNF-beta, whose cDNA is not yet cloned. We also analysed the upstream sequences of the rabbit TNF-beta and TNF-alpha genes and identified a number of potential binding sites for known nuclear transcription factors, and in particular several putative kappa B-type sequences.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Consensus Sequence
  • Gene Library
  • Genes
  • Humans
  • Lymphotoxin-alpha / genetics*
  • Molecular Sequence Data
  • Rabbits
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Sequence Alignment
  • Transcription Factors / metabolism
  • Tumor Necrosis Factor-alpha / genetics*

Substances

  • Lymphotoxin-alpha
  • Transcription Factors
  • Tumor Necrosis Factor-alpha

Associated data

  • GENBANK/M35236
  • GENBANK/M35237
  • GENBANK/M35326
  • GENBANK/M35327
  • GENBANK/M60340
  • GENBANK/M60341