Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry

Narasimha V Hegde; Bhushan M Jayarao; Chitrita DebRoy

doi:10.1128/JCM.00137-12

Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry

J Clin Microbiol. 2012 Jun;50(6):2137-9. doi: 10.1128/JCM.00137-12. Epub 2012 Apr 4.

Authors

Narasimha V Hegde¹, Bhushan M Jayarao, Chitrita DebRoy

Affiliation

¹ Escherichia coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA.

Abstract

Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10(3) target cells in a bacterial mixture of 10(5) CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteriological Techniques / methods*
Cattle
Flow Cytometry / methods*
Meat / microbiology*
Sensitivity and Specificity
Shiga-Toxigenic Escherichia coli / isolation & purification*
Time Factors