Staining membrane-bound proteins with colloidal gold

Wayne R Stochaj; Tom Berkelman; Nancy Laird

doi:10.1101/pdb.prot4545

Staining membrane-bound proteins with colloidal gold

CSH Protoc. 2006 Oct 1;2006(5):pdb.prot4545. doi: 10.1101/pdb.prot4545.

Authors

Wayne R Stochaj, Tom Berkelman, Nancy Laird

PMID: 22485940
DOI: 10.1101/pdb.prot4545

Abstract

INTRODUCTIONColloidal Gold is the most sensitive staining technique for proteins bound on membranes, detecting as little as 1-3 ng of protein. Protein spots are permanently stained a dark red after incubation with the Colloidal Gold solution. Colloidal Gold staining can detect proteins on both nitrocellulose and PVDF membranes, but it is not recommended for nylon membranes.