Staining membrane-bound proteins with coomassie blue r250

Wayne R Stochaj; Tom Berkelman; Nancy Laird

doi:10.1101/pdb.prot4544

Staining membrane-bound proteins with coomassie blue r250

CSH Protoc. 2006 Oct 1;2006(5):pdb.prot4544. doi: 10.1101/pdb.prot4544.

Authors

Wayne R Stochaj, Tom Berkelman, Nancy Laird

PMID: 22485939
DOI: 10.1101/pdb.prot4544

Abstract

INTRODUCTIONCoomassie Blue R250 permanently stains membrane-bound proteins and is compatible with PVDF and nitrocellulose membranes, but it is incompatible with nylon membranes. This technique is relatively insensitive, with a detection limit of ~1.5 μg of protein. One drawback of Coomassie Blue staining is that it produces a high background that can make interpretation of results difficult.