Magnetic resonance imaging of endothelial cells with vectorized iron oxide nanoparticles

Bull Exp Biol Med. 2011 Oct;151(6):726-30. doi: 10.1007/s10517-011-1426-5.

Abstract

We propose a method for obtaining superparamagnetic nanoparticles based on iron oxide and their water suspensions. The structure and size of nanoparticles were confirmed by transmission electron microscopy, dynamic light scattering, and X-ray diffraction analysis. The nanoparticles also contained a fluorescent dye Dil C18. Cytotoxicity of obtained aqueous suspension was studied by MTT assay; low toxicity of nanoparticles was demonstrated. High T2-relaxivity of nanoparticles allows using them as a contrast agent for MRI. After incubation of cerebellar sections with nanoparticles vectorized with antibodies to antigen AMVB1, specific visualization of blood vessels was detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Contrast Media / chemistry
  • Ferric Compounds / chemistry*
  • Magnetic Resonance Imaging / methods*
  • Microscopy, Electron, Transmission
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • X-Ray Diffraction

Substances

  • Contrast Media
  • Ferric Compounds
  • ferric oxide