The bacterium Clostridium difficile is pathogenic for both humans and animals. This feared nosocomial infectious agent is increasingly more important in the community as well. Like in humans, the gastrointestinal tract is infected in animals. The bacterium may be transmitted from animals to humans via the food chain (e.g. pork meat) or by direct contact. Identical human and animal isolates of C. difficile have been reported in several studies. The article presents results from a small local study aimed at detecting C. difficile and its toxins in the District of Olomouc, Czech Republic, by 3 different methods.