Abstract
This study used a replicative lifespan assay of K6001 yeast to screen anti-aging food factors in commercial flavonoids. Hesperidin derived from the Citrus genus extended the lifespan of yeast at doses of 5 and 10 µM as compared with the control group (p<0.01, p<0.01). Reactive oxygen species (ROS), real-time PCR (RT-PCR), and lifespan assays of uth1 and skn7 mutants with the K6001 background were used to study the anti-aging mechanisms in yeast. The results indicate that hesperidin significantly inhibits the ROS of yeast, and UTH1 gene expression, and that SKN7 gene are involved in hesperidin-mediated lifespan extension. Further, increases in the Sir2 homolog, SIRT1 activity, and SOD gene expression were confirmed at doses of 5 (p<0.01) and 10 µM (p<0.05). This suggests that Sir2, UTH1 genes, and ROS inhibition after administration of hesperidin have important roles in the anti-aging effects of yeast. However, the aglycon hesperetin did not exhibit anti-aging effects in yeast.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Flavonoids / pharmacology
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Gene Expression
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Heat-Shock Proteins / genetics*
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Heat-Shock Proteins / metabolism
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Hesperidin / pharmacology*
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mitochondrial Proteins / genetics*
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Mitochondrial Proteins / metabolism
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Oxidative Stress / drug effects
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Reactive Oxygen Species / antagonists & inhibitors*
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Real-Time Polymerase Chain Reaction
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Saccharomyces cerevisiae / drug effects*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae Proteins / metabolism
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Sirtuin 2 / genetics*
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Sirtuin 2 / metabolism
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Superoxide Dismutase / genetics*
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Superoxide Dismutase / metabolism
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Time Factors
Substances
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Flavonoids
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Heat-Shock Proteins
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Membrane Proteins
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Mitochondrial Proteins
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Reactive Oxygen Species
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Saccharomyces cerevisiae Proteins
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UTH1 protein, S cerevisiae
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Hesperidin
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Superoxide Dismutase
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Sirtuin 2
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hesperetin