Derivation of iPSCs in stirred suspension bioreactors

Nat Methods. 2012 Apr 8;9(5):465-6. doi: 10.1038/nmeth.1973.

Abstract

Induced pluripotent stem cells (iPSCs) are typically derived in adherent culture. Here we report fast and efficient derivation of mouse iPSCs in stirred suspension bioreactors, with and without the use of c-Myc. Suspension-reprogrammed cells expressed pluripotency markers, showed multilineage differentiation in vitro and in vivo, and contributed to the germline in chimeric mice. Suspension reprogramming has the potential to accelerate and standardize iPSC research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bioreactors
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cell Differentiation / physiology
  • Chimera / genetics
  • Flow Cytometry
  • Immunophenotyping / methods
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Proto-Oncogene Proteins c-myc / metabolism
  • Transfection / methods

Substances

  • Myc protein, mouse
  • Proto-Oncogene Proteins c-myc