A fluorescence-based analysis of aristolochic acid-derived DNA adducts

Anal Biochem. 2012 Aug 1;427(1):49-51. doi: 10.1016/j.ab.2012.03.027. Epub 2012 Apr 4.

Abstract

Aristolochic acids (AAs), major components of plant extracts from Aristolochia species, form (after metabolic activation) pro-mutagenic DNA adducts in renal tissue. The DNA adducts can be used as biomarkers for studies of AA toxicity. Identification of these adducts is a complicated and time-consuming procedure. We present here a fast, nonisotopic, fluorescence-based assay for the detection of AA-DNA adducts in multiple samples. This approach allows analysis of AA adducts in synthetic DNA with known nucleotide composition and analysis of DNA adducts formed from chemically diverse AAs in vitro. The method can be applied to compare AA-DNA adduct formation in cells and tissues.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Aristolochia / chemistry*
  • Aristolochic Acids* / chemistry
  • Aristolochic Acids* / pharmacology
  • Aristolochic Acids* / toxicity
  • Chromatography, High Pressure Liquid
  • DNA Adducts / analysis*
  • DNA Adducts / chemistry
  • DNA Adducts / drug effects
  • DNA Damage / drug effects
  • DNA* / chemical synthesis
  • DNA* / chemistry
  • Fluorescence
  • LLC-PK1 Cells
  • Mutagens / chemistry
  • Mutagens / pharmacology
  • Plant Extracts* / chemistry
  • Plant Extracts* / pharmacology
  • Swine

Substances

  • Aristolochic Acids
  • DNA Adducts
  • Mutagens
  • Plant Extracts
  • DNA
  • aristolochic acid I