Negative staining of lipopolysaccharides on polyacrylamide gels by using eosin B

Zhong-Xin Zhu; Wei-Tao Cong; Xuan Zhou; Mao Chen; Jung-Kap Choi; Li-Tai Jin; Xiao-Kun Li

doi:10.1016/j.ab.2012.03.022

Negative staining of lipopolysaccharides on polyacrylamide gels by using eosin B

Anal Biochem. 2012 Jul 1;426(1):1-3. doi: 10.1016/j.ab.2012.03.022. Epub 2012 Apr 4.

Authors

Zhong-Xin Zhu¹, Wei-Tao Cong, Xuan Zhou, Mao Chen, Jung-Kap Choi, Li-Tai Jin, Xiao-Kun Li

Affiliation

¹ Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou 325035, China.

PMID: 22484039
DOI: 10.1016/j.ab.2012.03.022

Abstract

A sensitive and simple technique for the negative detection of lipopolysaccharides (LPSs) following polyacrylamide gel electrophoresis (PAGE) using eosin B (EB) was developed. After electrophoresis, gels were fixed, stained, and developed within 30 min to achieve transparent and colorless LPS bands under opaque gel matrix background. As low as 20 to 40 ng of total LPSs could be detected, which is 4-fold more sensitive than those of the widely used silver stain developed by Fomsgaard and coworkers and imidazole-zinc (IZ) negative stain. For its sensitivity and brevity, this stain may be a practical method for LPS determination in the routine laboratory.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Polyacrylamide Gel*
Eosine I Bluish
Fluoresceins / chemistry*
Lipopolysaccharides / analysis*
Negative Staining / methods*

Substances

Fluoresceins
Lipopolysaccharides
Eosine I Bluish