Super-resolved spatial light interference microscopy

J Opt Soc Am A Opt Image Sci Vis. 2012 Mar 1;29(3):344-51. doi: 10.1364/JOSAA.29.000344.

Abstract

We report a scheme to achieve resolution beyond the diffraction limit in spatial light interference microscopy (SLIM). By adding a grating to the optical path, the structured illumination technique can be used to improve the resolution by a factor of 2. We show that a direct application of the structured illumination technique, however, has proved to be unsuccessful. Through two crucial modifications, namely, one to the pupil plane of the objective and the other to the demodulation procedure, faithful phase information of the object is recovered and the resolution is improved by a factor of 2.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Fourier Analysis
  • Light*
  • Microscopy, Interference / methods*
  • Quality Control