Live imaging of the lung

Curr Protoc Cytom. 2012 Apr:Chapter 12:12.28.1-12.28.12. doi: 10.1002/0471142956.cy1228s60.

Abstract

Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion incurred by ventilation and cardiac contractions. In this chapter, we report protocols for imaging ex vivo live lung slices and the intact mouse lung.

MeSH terms

  • Animals
  • Cell Movement
  • Diagnostic Imaging / methods*
  • Fluorescent Antibody Technique
  • Green Fluorescent Proteins / metabolism
  • Instillation, Drug
  • Lung / anatomy & histology*
  • Lung / cytology
  • Lung / surgery
  • Mice
  • Microscopy
  • Staining and Labeling

Substances

  • enhanced green fluorescent protein
  • Green Fluorescent Proteins