The isolation of single cells from the avian salt gland

Am J Anat. 1979 Jun;155(2):281-6. doi: 10.1002/aja.1001550209.

Abstract

Collagenase, hyaluronidase, and trypsin were used to isolate single cells from the avian salt gland. Three cell types were distinquishable in the resultant cell suspension: peripheral, intermediate, and principal cells. The fine structure of these cells is described and related to the morphology of the intact gland.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Membrane / ultrastructure
  • Cell Separation
  • Ducks / anatomy & histology*
  • Hyaluronoglucosaminidase
  • Microbial Collagenase
  • Mitochondria / ultrastructure
  • Ruthenium Red
  • Salt Gland / cytology*
  • Trypsin

Substances

  • Ruthenium Red
  • Hyaluronoglucosaminidase
  • Trypsin
  • Microbial Collagenase