The effect of a continuous-wave (cw) laser based anemometer's probe volume on the measurement of wind turbulence is studied in this paper. Wind speed time series acquired by both a remote sensing cw laser anemometer, whose line-of-sight was aligned with the wind direction, and by a reference sensor (sonic anemometer) located in the same direction, were used. The spectral transfer function, which describes the attenuation of the power spectral density of the wind speed turbulence, was calculated and found to be in good agreement with the theoretical exponential function, which is based on the properties of the probe volume of a focused Gaussian laser beam. Parameters such as fluctuations of the wind direction, as well as the overestimation of the laser Doppler spectrum threshold, were found to affect the calculation of the spectral transfer function by introducing high frequency noise.