Ultrasensitive colorimetric DNA detection using a combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA)

Small. 2012 Jun 25;8(12):1846-50. doi: 10.1002/smll.201200263. Epub 2012 Mar 28.

Abstract

A combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary sequences at ultralow concentrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Colorimetry / methods*
  • DNA / analysis*
  • DNA / chemistry
  • Endonucleases / metabolism
  • Escherichia coli / metabolism
  • Gold / chemistry
  • Metal Nanoparticles / chemistry
  • Microscopy, Atomic Force
  • Molecular Sequence Data
  • Nanoparticles / chemistry*
  • Nucleic Acid Amplification Techniques
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemistry
  • Sequence Homology, Nucleic Acid

Substances

  • Oligonucleotides
  • Gold
  • DNA
  • Endonucleases