Abstract
Recombinant human cyclooxygenase 1 (COX-1) was expressed from stably-transfected Drosophila melanogaster S2 (S2) cells. DMSO improved the expression of recombinant COX-1 by 180 %. DMSO increased the expression of nitric oxide synthase (NOS) at both the RNA and protein levels; NOS expression was closely correlated with the synthesis of recombinant COX-1 mRNA in stably-transfected S2 cells. DMSO also induced the gene encoding Kr-h1 which binds to the CACCC element of the metallothionein promoter to enhance the expression of recombinant COX-1. Therefore, DMSO improves the expression of recombinant COX-1 via NOS and/or the transcription factor Kr-h1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Cell Line
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Cyclooxygenase 1 / biosynthesis*
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Cyclooxygenase 1 / genetics
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Dimethyl Sulfoxide / metabolism*
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Drosophila Proteins / metabolism*
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Drosophila melanogaster
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Gene Expression
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Gene Expression Profiling
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Gene Expression Regulation / drug effects*
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Humans
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Kruppel-Like Transcription Factors / metabolism*
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Nitric Oxide Synthase / biosynthesis*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Transfection
Substances
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Drosophila Proteins
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Kr-h1 protein, Drosophila
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Kruppel-Like Transcription Factors
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Recombinant Proteins
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Nitric Oxide Synthase
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Cyclooxygenase 1
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Dimethyl Sulfoxide