Abstract
Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Catalytic Domain*
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Click Chemistry
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Endopeptidases / chemistry*
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Endopeptidases / metabolism*
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Enzyme Assays / methods*
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism*
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HEK293 Cells
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Humans
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Models, Molecular
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Ubiquitin / chemistry
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Ubiquitin / metabolism
Substances
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Fluorescent Dyes
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Ubiquitin
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Endopeptidases