Separation and identification of rice prolamins by two-dimensional gel electrophoresis and amino acid sequencing

Takanari Shigemitsu; Yuhi Saito; Shigeto Morita; Shigeru Satoh; Takehiro Masumura

doi:10.1271/bbb.110791

Separation and identification of rice prolamins by two-dimensional gel electrophoresis and amino acid sequencing

Biosci Biotechnol Biochem. 2012;76(3):594-7. doi: 10.1271/bbb.110791.

Authors

Takanari Shigemitsu¹, Yuhi Saito, Shigeto Morita, Shigeru Satoh, Takehiro Masumura

Affiliation

¹ Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto, Japan.

PMID: 22451407
DOI: 10.1271/bbb.110791

Abstract

There are difficulties in detecting and separating rice prolamin polypeptides by 2D-PAGE analysis because prolamin polypeptides are insoluble, and the amino acid sequences show high homology among them. In this study, we improved the prolamin extraction method and the 2D-PAGE procedure, and succeeded in separating prolamin polypeptide species by 2D-PAGE and in identifying major prolamin polypeptide sequences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Electrophoresis, Gel, Two-Dimensional*
Oryza / chemistry*
Prolamins / analysis
Prolamins / chemistry*
Prolamins / isolation & purification*
Sequence Analysis*

Substances

Prolamins