Abstract
In the present investigation, the abundance and molecular phylogeny of part of the culturable bacterial population involved in the dissolution of "miliolite", a bioclastic limestone, from Gopnath, India, was studied. Carbonate-dissolving bacteria were isolated, enumerated and screened for their ability to dissolve miliolite. Amplified ribosomal DNA restriction analysis (ARDRA) indicated 14 operational taxonomic units (OTUs) to be distributed in 5 different clades at a similarity coefficient of 0.85. Then, 16S rRNA sequence analysis helped to decipher that the majority of carbonate-dissolving bacteria were affiliated to phyla Firmicutes (Families Bacillaceae and Staphylococcaceae) and Actinobacteria (Family Promicromonosporaceae) indicating their role in miliolite weathering.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacteria / classification*
-
Bacteria / isolation & purification*
-
Bacteria / metabolism
-
Calcium Carbonate*
-
Carbonates / metabolism*
-
Cluster Analysis
-
DNA Fingerprinting
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / genetics
-
DNA, Ribosomal / chemistry
-
DNA, Ribosomal / genetics
-
Environmental Microbiology*
-
India
-
Molecular Sequence Data
-
Phylogeny
-
RNA, Ribosomal, 16S / genetics
-
Sequence Analysis, DNA
Substances
-
Carbonates
-
DNA, Bacterial
-
DNA, Ribosomal
-
RNA, Ribosomal, 16S
-
Calcium Carbonate
Associated data
-
GENBANK/JN092561
-
GENBANK/JN092562
-
GENBANK/JN092563
-
GENBANK/JN092564
-
GENBANK/JN092565
-
GENBANK/JN092566
-
GENBANK/JN092567
-
GENBANK/JN092568
-
GENBANK/JN092569
-
GENBANK/JN092570
-
GENBANK/JN092571
-
GENBANK/JN092572
-
GENBANK/JN092573
-
GENBANK/JN092574