Abstract
Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-β) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-β, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-β, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amnion / cytology
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Amnion / immunology*
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Cell Differentiation / immunology
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Coculture Techniques
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Dinoprostone / genetics
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Dinoprostone / immunology
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Female
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Hepatocyte Growth Factor / genetics
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Hepatocyte Growth Factor / immunology
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Humans
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Immunologic Factors / immunology*
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Immunophenotyping
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Indoleamine-Pyrrole 2,3,-Dioxygenase / genetics
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Indoleamine-Pyrrole 2,3,-Dioxygenase / immunology
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Interferon-gamma / immunology
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Interleukin-10 / analysis
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Interleukin-10 / immunology
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Interleukin-17 / analysis
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Interleukin-17 / immunology
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / immunology
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / immunology*
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Pregnancy
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RNA, Messenger / chemistry
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RNA, Messenger / genetics
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Regenerative Medicine / methods
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Reverse Transcriptase Polymerase Chain Reaction
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / immunology
Substances
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Immunologic Factors
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Indoleamine-Pyrrole 2,3,-Dioxygenase
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Interleukin-17
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RNA, Messenger
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Transforming Growth Factor beta
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Interleukin-10
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Hepatocyte Growth Factor
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Interferon-gamma
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Dinoprostone